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Progressive Tactile Perception and Peripheral Hemodynamic Responses Induced by LIFUS on Fingertip

Liuni Qin, Yinshen Huang, Jin Xie, Lili Niu, Laixin Huang, Fei Li, Shichun Bao, Guanglin Li, Yanjuan Geng

IEEE Transactions on Neural Systems and Rehabilitation Engineering 2026, 34, 1305-1317 · 10.1109/tnsre.2026.3664418

human healthyhealthybehaviourother mri

Abstract

Peripherally applied low-intensity focused ultrasound stimulation (LIFUS) has emerged as a new modality of tactile restoration recently. If repetitive LIFUS would cause perceptual adaptation, like transcutaneous electrical nerve stimulation (TENS) does, has been rarely investigated. To address this issue, 14 healthy volunteers received LIFUS-based fine tactile stimulation on their right index fingertip in this work. To evaluate their perceptual stability, both subjective perceptual ratings and peripheral local hemodynamic responses were deployed. The sensory-level TENS was also included for comparison. Our results showed that the LIFUS brought better perceptual acuity and perceptual stability than TENS in terms of subjective perception and judgement. Moreover, the LIFUS induced an increase of local blood perfusion volume (BPV) since stimulation onset, while the TENS caused a decrease of BPV, both followed by a slow rebound to the baseline. Notably, repetitive LIFUS didn't cause obvious progressive decrease of BPV responses with increasing dose, i.e., temporal accumulation, whereas TENS did. These findings would facilitate the development of non-invasive sensory feedback technique in multiple human-machine interaction scenarios, and shed valuable insights on neuromodulation mechanisms of peripherally applied LIFUS.

Abstract via europepmc.

Specieshuman
Subjects16 participants
Sessions per subject2
Randomisednot reported
Blindingnot reported
Sham / controlnone
Auditory controlmasking sound
Readout timingonline
Anaesthesianot applicable
Readoutsbehaviour, other mriSubjective tactile-sensation discrimination accuracy (tapping/vibration/electrical/pressure/no-stimulation); local blood perfusion volume (BPV) measured by laser speckle imaging
Direction of effectexcitatoryLIFUS evoked distinguishable fine tactile sensations with high discrimination accuracy and produced an increase in local blood perfusion volume (vasodilation) beneath the fingertip since stimulation onset, unlike TENS which decreased BPV.
Adverse eventsobservedSome participants reported nociceptive pain (burning/twinge) with inappropriate parameters (AI above 1.96 MPa or PRF above 100 Hz) or after repeated strong stimulation during pretesting; affected participants were excluded or rescheduled. Estimated and measured focal temperature rises were 0.76C and 0.72C respectively, below levels expected to cause pain. Follow-up at one week and two months found no short- or long-term adverse reaction.

Exposures

Exposure 1: LIFUS-250

Target: digital nerve — “right index fingertip
Device: custom-built · Pantsd Inc. · HN81C-750-250KC

Pulse timing
Waveformpulsed
Fundamental frequency (kHz)250✓✓
Pulse duration (ms)not reported
Pulse repetition frequency (Hz)4, 7, 14, 24, 63, 72, 80, 88swept✓✓
Duty cycle (%)3✓✓
Sonication duration (s)2✓✓
Pressure and intensity, by domain
Free-field pressure (kPa)not reported
Free-field Isppa (W/cm²)10.28, 12.26, 22.82, 25.92, 10.28, 13.91, 20.86, 28.02swept?
Free-field Ispta (W/cm²)0.25, 0.37, 0.69, 0.78, 0.22, 0.42, 0.63, 0.84swept?
In-situ estimatenot reported
In-situ pressure (kPa)not reported
In-situ Isppa (W/cm²)not reported
In-situ Ispta (W/cm²)not reported
Protocol, in the paper’s words

Four distinct PRF ranges (4-7, 14-24, 63-72, 80-88 Hz) were paired with a moderate or strong acoustic intensity (AI, 30-40% or 60-70% of each participant's individually determined [AImin, AImax] threshold range) to elicit tapping, vibration, electrical, and pressure sensations, respectively, each delivered for a 2-s trial. A separate pretest to determine AI thresholds used a fixed PRF of 50 Hz, a fixed duty cycle of 3%, and a 2-s stimulation duration; duty cycle for the four main sensation-eliciting conditions is not stated.

Exposure 2: LIFUS-500

Target: digital nerve — “right index fingertip
Device: custom-built · NDT Inc. · IBP0.512

Pulse timing
Waveformpulsed
Fundamental frequency (kHz)500✓✓
Pulse duration (ms)not reported
Pulse repetition frequency (Hz)4, 7, 14, 24, 63, 72, 80, 88swept✓✓
Duty cycle (%)3✓✓
Sonication duration (s)2✓✓
Pressure and intensity, by domain
Free-field pressure (kPa)not reported
Free-field Isppa (W/cm²)10.28, 16.36, 31.51, 35.33, 12.78, 15.03, 31.68, 35.33swept?
Free-field Ispta (W/cm²)0.31, 0.49, 0.95, 1.06, 0.38, 0.45, 1.04, 1.15swept?
In-situ estimatenot reported
In-situ pressure (kPa)not reported
In-situ Isppa (W/cm²)not reported
In-situ Ispta (W/cm²)not reported
Protocol, in the paper’s words

Four distinct PRF ranges (4-7, 14-24, 63-72, 80-88 Hz) were paired with a moderate or strong acoustic intensity (AI, 30-40% or 60-70% of each participant's individually determined [AImin, AImax] threshold range) to elicit tapping, vibration, electrical, and pressure sensations, respectively, each delivered for a 2-s trial. A separate pretest to determine AI thresholds used a fixed PRF of 50 Hz, a fixed duty cycle of 3%, and a 2-s stimulation duration; duty cycle for the four main sensation-eliciting conditions is not stated.

Flags from extraction

  • n_subjectsAbstract states '14 healthy volunteers' but Methods states '16 healthy right-handed volunteers ... were recruited'; the more detailed Methods count (16) is used.
  • exposures[0].timing.duty_cycle_pctA fixed duty cycle of 3% is stated only for the separate AI-threshold pretest (PRF 50 Hz, 2 s); duty cycle for the four main sensation-eliciting stimuli (Table I) is not stated.
  • exposures[0].timing.pulse_duration_msNot stated; only PRF and intensity are given for the sensation-eliciting stimuli, and duty cycle needed to derive pulse duration is not reported for these conditions.
  • exposures[0].timing.pulse_repetition_frequency_hzTable I gives four separate PRF ranges (one per tactile sensation type); recorded as the flattened list of the eight range boundaries in table order (tapping, vibration, electrical, pressure).
  • exposures[0].free_field.isppa_w_cm2Isppa/Ispta were measured with a needle hydrophone in a degassed water bath (device characterization), then delivered directly onto the acoustically transparent skin with no intervening skull; classified as free-field, and recorded as the eight table values (one per sensation type/level) in table order.
  • auditory_controlParticipants wore eye masks and earmuffs during testing to avoid visual and auditory distraction; classified as masking_sound.
  • sham_typeA 'no stimulation' condition was included as one of the five randomly-ordered trial types within each block, functioning as a within-session no-stimulation control; classified as none because no separate sham transducer/control arm is described.