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Deep transcranial ultrasound stimulation using personalized acoustic metamaterials improves treatment-resistant depression in humans

David Attali, Thomas Tiennot, Thomas J. Manuel, Maxime Daniel, Alexandre Houdouin, Philippe Annic, Alexandre Dizeux, Alexandre Haroche, Ghita Dadi, Adèle Henensal, Mylène Moyal, Alice Le Berre, Cécile Paolillo, Sylvain Charron, Clément Debacker, Maliesse Lui, Sabrina Lekcir, Rosella Mancusi, Thierry Gallarda, Tarek Sharshar, Khaoussou Sylla, Catherine Oppenheim, Arnaud Cachia, Mickael Tanter, Jean-Francois Aubry, Marion Plaze

Brain Stimulation 2025, 18, 1004-1014 · 10.1016/j.brs.2025.04.018

human patientdepressionclinical scalefmriother mribehaviour

Abstract

Background Neuromodulation of deep brain regions has shown promise for treatment-resistant depression (TRD). However, it currently requires neurosurgical electrode implantation, posing significant risks and limiting widespread use while TRD affects around 100 million people worldwide. Low-intensity transcranial ultrasound stimulation (TUS) could allow precise and non-invasive deep neuromodulation, provided that the challenge of the defocusing effects of the skull is tackled. Objective/hypothesis Here, we present the development of a portable and neuronavigated TUS prototype based on the use of patient-specific metamaterials (metalens) that correct for skull-induced aberrations. We then present the first application of metalens-based Transcranial Ultrasound Stimulation (mTUS) in TRD. The primary objective was to assess the safety and efficacy of mTUS targeting on individual level specific white matter tracts of the subcallosal cingulate involved in TRD. Methods The safety and precision of this device was addressed through a series of numerical simulations and experimental measurements on ex vivo human skulls. Five participants with TRD were included in this open-label study (ClinicalTrials.gov identifier: NCT06085950) and underwent an intensive 5-day course of mTUS with a total of 25 sessions of 5 min each. Results No serious adverse events occurred during the study. By day 5 of treatment, depression severity was reduced by an average of 60.9 % (range: [30 %-83.9 %]), and four out of five patients qualified as responders, with two of them in remission. Conclusions This study provides first-in-human evidence of the potential of mTUS as a precise, safe and effective non-invasive neuromodulation technique for neuropsychiatric disorders involving deep brain regions, offering a safer and more accessible alternative to invasive approaches.

Abstract via europepmc.

Specieshuman
Subjects5 participants
Sessions per subject25
Randomisedno
Blindingnone
Sham / controlnone
Auditory controlramped pulses
Readout timingoffline
Anaesthesianot applicable
Readoutsclinical scale, fmri, other mri, behaviourMADRS; 17- and 6-item HDRS; QIDS-SR; neuropsychological battery (D2 test of attention, trail making test, category fluency task, Grober & Buschke test, WAIS-IV digit span, Stroop test); resting-state fMRI functional connectivity of the left SCC; T1/FLAIR/T2* MRI for safety
Direction of effectinhibitoryA low duty cycle protocol associated with inhibitory stimulation was chosen to inhibit the hyperactive SCC in depression; depression severity decreased over the treatment course, and exploratory fMRI showed altered SCC connectivity with left DLPFC (increased) and right hippocampus/parahippocampus (decreased).
Adverse eventsobservedNo serious adverse events occurred; mild transient unintended effects (sleepiness, anxiety/nervousness, tinnitus, fatigue, tingling, burning/heat, euphoria, transducer pressure) were reported after a minority of sessions and resolved within days.

Exposures

Exposure 1: mTUS to left subcallosal cingulate (SCC)

Target: subgenual anterior cingulate cortex — “left subcallosal cingulate (SCC), at the crossing of the forceps minor, cingulum bundle and uncinate fasciculus
Device: IGT / Imasonic · Imasonic (transducer); Image Guided Therapy (driving electronics) · Serial Number 13474-1002

Pulse timing
Waveformpulsed
Fundamental frequency (kHz)500✓✓
Pulse duration (ms)4.5✓✓
Pulse repetition frequency (Hz)14✓✓
Duty cycle (%)5pulse duration × PRF gives 6.3%, which disagrees with the stated value✓✓
Sonication duration (s)5✓✓
Pressure and intensity, by domain
Free-field pressure (kPa)not reported
Free-field Isppa (W/cm²)not reported
Free-field Ispta (W/cm²)not reported
In-situ estimatederatingmean or range across subjects
In-situ pressure (kPa)not reported
In-situ Isppa (W/cm²)not reported
In-situ Ispta (W/cm²)0.184✓✓
Protocol, in the paper’s words

The electronics were programmed to deliver ramped pulses with a 5% duty cycle (pulse duration of 4.5 ms including ramp duration of 0.75 ms, pulse repetition interval of 71 ms, pulse repetition frequency of 14 Hz), repeated in a pulse train (pulse train duration of 5 s, pulse train repetition interval of 15 s, pulse train repetition frequency of 0.07 Hz, pulse train duty cycle of 33%), resulting in an overall duty cycle of 1.8%. The 5-s pattern was repeated 20 times for a total treatment time of 5 min. Pulsed ultrasound was applied for 5 min, 5 times per day for 5 days with a minimum delay of 1 h between two sessions.

Flags from extraction

  • exposures[0].in_situ.ispta_w_cm2Paper reports derated ISPTA both without correction (91.7 mW/cm2 mean) and with the personalized metalens (184.4 mW/cm2 mean) in the same sentence; the metalens value (as actually used clinically) is recorded here.
  • exposures[0].timing.duty_cycle_pctStated pulse duty cycle is 5%, but pulse duration (4.5 ms) x PRF (14 Hz) = 6.3%, a minor discrepancy possibly reflecting rounding of the ramp duration; recorded as stated (5%).
  • randomisedOpen-label single-arm pilot trial; no randomisation to treatment arms was performed.