Transcranial focused ultrasound induces sustained synaptic plasticity in rat hippocampus

Abstract

Transcranial focused ultrasound (tFUS) neuromodulation provides a promising emerging non-invasive therapy for the treatment of neurological disorders. Many studies have demonstrated the ability of tFUS to elicit transient changes in neural responses. However, the ability of tFUS to induce sustained changes need to be carefully examined. In this study, we use the long-term potentiation/long term depression (LTP/LTD) model in the rat hippocampus, the medial perforant path (mPP) to dentate gyrus (DG) pathway, to explore whether tFUS is capable of encoding frequency specific information to induce plasticity. Single-element focused transducers were used for tFUS stimulation with ultrasound fundamental frequency of 0.5 MHz and nominal focal distance of 38 mm tFUS stimulation is directed to mPP. Measurement of synaptic connectivity is achieved through the slope of field excitatory post synaptic potentials (fEPSPs), which are elicited using bipolar electrical stimulation electrodes and recorded at DG using extracellular electrodes to quantify degree of plasticity. We applied pulsed tFUS stimulation with total duration of 5 min, with 5 levels of pulse repetition frequencies each administered at 50 Hz sonication frequency at the mPP. Baseline fEPSP is recorded 10 min prior, and 30+ minutes after tFUS administration. In N = 16 adult wildtype rats, we observed sustained depression of fEPSP slope after 5 min of tFUS focused at the presynaptic field mPP. Across all PRFs, no significant difference in maximum fEPSP slope change was observed, average tFUS induced depression level was observed at 19.6%. When compared to low frequency electrical stimulation (LFS) of 1 Hz delivered to the mPP, the sustained changes induced by tFUS stimulation show no statistical difference to LFS for up to 24 min after tFUS stimulation. When both the maximum depression effects and the duration of sustained effects are both taken into account, PRF 3 kHz can induce significantly larger effects than other PRFs tested. tFUS stimulation is measured with a spatial-peak pressure amplitude of 99 kPa, translating to an estimation of 0.43 °C temperature increase when assuming no loss of heat. The results suggest the ability of tFUS to encode sustained changes in synaptic connectivity through mechanism which are unlikely to involve thermal changes.

Abstract via europepmc.

Flags from extraction

• exposures[0].timing.pulse_repetition_frequency_hz — Paper states a range (3-10 kHz) for '5 different tFUS PRFs tested' but does not list all 5 exact values in the main text (only 3, 4, and 10 kHz are named in specific comparisons); range endpoints recorded.
• exposures[0].timing.pulse_duration_ms — Stimulation has a nested burst structure (individual PRF pulses at 50% duty cycle, further grouped into 10-ms-on/10-ms-off blocks at a 50 Hz 'encoding frequency'); the paper gives an example pulse duration of '167 ms' for PRF 3000 Hz, which appears to be a units typo for microseconds (83 cycles at 500 kHz = ~166 us, not ms). Left not_reported and described in protocol_description per the burst rule.
• exposures[0].free_field.isppa_w_cm2 — Value is stated only in a figure legend (Fig. 1E-F caption), not the main narrative text.
• exposures[0].free_field.ispta_w_cm2 — Value is stated only in a figure legend (Fig. 1E-F caption), not the main narrative text.
• n_sessions_per_subject — Paper states 'For each animal, one to two sham conditions, and five to six tFUS conditions were applied', a range rather than a fixed number of sessions per subject.