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Transcranial Focused Ultrasound Enhances Sensory Discrimination Capability through Somatosensory Cortical Excitation

Chang Liu, Kai Yu, Xiaodan Niu, Bin He

Ultrasound in Medicine & Biology 2021, 47, 1356-1366 · 10.1016/j.ultrasmedbio.2021.01.025

human healthyhealthyeeg megbehaviour

Abstract

Low-intensity transcranial focused ultrasound (tFUS) has emerged as a non-invasive brain neuromodulation tool with high spatial specificity. Previous studies attributed tFUS-enhanced sensory performance to the ultrasound-induced inhibitory neural effects. However, to date there is no direct evidence validating the neural mechanism underlying ultrasound-mediated somatosensory enhancement. In this study, healthy human subjects (N = 9) were asked to perform tactile vibration frequency discrimination tasks while tFUS was directed onto the primary somatosensory cortex. During this task, we simultaneously recorded 64-channel electroencephalography (EEG) signals and investigated the brain responses at both EEG sensors and source domains by means of electrophysiological source imaging (ESI). The behavioral results indicated that the subjects' discrimination ability was improved by tFUS with an increased percentage of correct responses. EEG and ESI results revealed that tFUS neuromodulation was able to improve sensory discrimination capability through excitatory effects at the targeted sensory cortex.

Abstract via europepmc.

Specieshuman
Subjects9 participants
Sessions per subject1
Randomisedyes
Blindingnot reported
Sham / controlsound only
Auditory controlsound only sham
Readout timingboth
Anaesthesianot applicable
Readoutseeg meg, behavioursomatosensory evoked potentials (SEP) via 64-channel EEG and electrophysiological source imaging (ESI); tactile vibration frequency discrimination task (percentage of responses correct, PRC)
Direction of effectexcitatorytFUS at UPRF 300 Hz increased early SEP components (N30, P100-N170) and somatosensory source profile amplitude at S1, and improved vibration frequency discrimination accuracy (PRC), interpreted as excitatory neuromodulation rather than the inhibitory effect reported by prior work.
Adverse eventsnot reported

Exposures

Exposure 1: tFUS to primary finger somatosensory cortex, UPRF 300 Hz

Target: primary somatosensory cortex — “primary finger somatosensory cortical area (S1)
Device: Blatek · Blatek Industries, Inc. · AT31529

Pulse timing
Waveformpulsed
Fundamental frequency (kHz)500✓✓
Pulse duration (ms)0.2✓✓
Pulse repetition frequency (Hz)300✓✓
Duty cycle (%)not reportedpulse duration × PRF gives 6%
Sonication duration (s)0.5✓✓
Pressure and intensity, by domain
Free-field pressure (kPa)780.4✓✓
Free-field Isppa (W/cm²)5.64✓✓
Free-field Ispta (W/cm²)0.338✓✓
In-situ estimatemeasurementsingle value
In-situ pressure (kPa)286✓✓
In-situ Isppa (W/cm²)1.1✓✓
In-situ Ispta (W/cm²)0.0671✓✓
Protocol, in the paper’s words

Pulsed US (100 cycles per pulse at 0.5 MHz fundamental frequency) was delivered to the finger representation area of S1 at 100 ms before the onset of each vibration stimulus, with each sonication lasting 500 ms at a UPRF of 300 Hz. A sham US session used active acoustic transmission but with the transducer physically decoupled from the scalp by 4-6 cm, serving as both sham and auditory/EMI confound control. Session order (sham vs. UPRF 300 Hz) was randomized.

Consistency checks: intensity pressure inconsistent free field; intensity pressure inconsistent in situ.

Flags from extraction

  • exposures[0].timing.pulse_duration_mspulse duration (0.2 ms) computed from the paper's own stated cycles-per-pulse (100) divided by fundamental frequency (0.5 MHz), a unit conversion sanctioned for this field.
  • exposures[0].unspecified_domain.pressure_kpathe 780.4 kPa value is described as 'applied to the scalp' (measured with a real skull sample present, not in free water alone, and not explicitly said to be in the brain), so its domain is ambiguous; placed in unspecified_domain rather than free_field or in_situ.
  • exposures[0].timing.duty_cycle_pctduty cycle not explicitly stated by the paper (only cycles-per-pulse and UPRF given); not computed.