Transcranial Ultrasound Stimulation Directly Influences the Cortical Excitability of the Motor Cortex in Parkinsonian Mice
Zhijie Wang, Jiaqing Yan, Xingrang Wang, Yi Yuan, Xiaoli Li
Movement Disorders 2020, 35, 693-698 · 10.1002/mds.27952
Abstract
Background Low-intensity transcranial ultrasound stimulation is a new noninvasive brain modulation method with high spatial resolution and high penetration depth. However, until now, it was unclear whether transcranial ultrasound stimulation has a significant effect on PD. Objectives In order to evaluate the effect of transcranial ultrasound stimulation on PD. Methods We used transcranial ultrasound stimulation to modulate parkinsonian-related activity in mice administered MPTP and recorded local field potentials in the motor cortex before and after ultrasound stimulation. We analyzed neuronal oscillatory activity known to be relevant to the pathophysiology of PD. Results After ultrasound stimulation, mean power intensity in the beta band (13-30 Hz) significantly decreased, and the phase-amplitude coupling strength between the beta and high gamma (55-100 Hz) bands and between the beta and ripple (100-200 Hz) bands also became significantly weaker. Conclusions This study demonstrates that ultrasonic neuromodulation can significantly decrease parkinsonian-related activity in mice administered MPTP. © 2019 International Parkinson and Movement Disorder Society.
Abstract via europepmc.
Exposures
Exposure 1: TUS targeting STN in MPTP-treated (parkinsonian) mice
Target: subthalamic nucleus — “STN”
Device: Olympus / Panametrics · Olympus America, Inc. · V301-SU ✓
| Waveform | pulsed | |
|---|---|---|
| Fundamental frequency (kHz) | 500 | ✓✓✓ |
| Pulse duration (ms) | not reportedimplied by duty cycle ÷ PRF: 0.05 ms (not stated by the paper) | |
| Pulse repetition frequency (Hz) | 1,000 | ✓✓✓ |
| Duty cycle (%) | 5 | ✓✓✓ |
| Sonication duration (s) | 0.05 | ✓✓✓ |
| Free-field pressure (kPa) | not reported | |
|---|---|---|
| Free-field Isppa (W/cm²) | not reported | |
| Free-field Ispta (W/cm²) | not reported | |
| In-situ estimate | not reported | |
| In-situ pressure (kPa) | not reported | |
| In-situ Isppa (W/cm²) | not reported | |
| In-situ Ispta (W/cm²) | not reported | |
| Pressure, domain unspecified (kPa) | 390 | ✓✓✓⚑ |
| Isppa, domain unspecified (W/cm²) | 5.1 | ✓✓✓ |
The ultrasound parameters fundamental frequency (FF), pulsed repetition frequency (PRF), duty cycle (DC), and stimulation duration (SD) were 500 kHz, 1 kHz, 5%, and 50 ms, respectively. The interstimulus interval was 1 second, and the total stimulation time was 5 minutes. In the PD + TUS group, we continuously recorded LFPs for 5 minutes and then stimulated the STN by TUS for 5 minutes (LFPs were not recorded during stimulation); finally, we recorded LFPs for another 5 minutes.
Flags from extraction
exposures[0].unspecified_domain.pressure_kpa— Paper does not state whether the 0.39 MPa / 5.1 W/cm2 field measurement (by needle hydrophone) was made in free field/water or in situ; domain left unspecified.n_subjects— N=11 is the PD+TUS group size (the only group receiving active TUS); healthy, PD, and PD+Sham groups (N=6 each, total 77 mice used study-wide across this and related work) were not counted.