Low-Intensity Pulsed Ultrasound Stimulation Induces Coupling Between Ripple Neural Activity and Hemodynamics in the Mouse Visual Cortex
Yi Yuan, Zhijie Wang, Xingran Wang, Jiaqing Yan, Mengyang Liu, Xiaoli Li
Cerebral Cortex 2019 · 10.1093/cercor/bhy187
Abstract
Several studies have separately investigated neural activities and hemodynamic responses induced by low-intensity pulsed ultrasound stimulation (LIPUS), less is known about their coupling under LIPUS. This study aims to investigate the neurovascular coupling with LIPUS by measuring neural activity and hemodynamics. We found that the relative power and sample entropy of local field potential at the ripple band have a significant correlation to relative cerebral blood flow over time (correlation coefficients: 0.66 ± 0.13 [P < 0.01] and -0.58 ± 0.11 [P < 0.05]). These results demonstrate that LIPUS can induce neurovascular coupling in the mouse visual cortex.
Abstract via europepmc.
Exposures
Exposure 1: LIPUS to visual cortex
Target: visual cortex — “visual cortex”
Device: Olympus / Panametrics · Olympus · V301-SU ✓
| Waveform | pulsed | |
|---|---|---|
| Fundamental frequency (kHz) | 500 | ✓✓✓ |
| Pulse duration (ms) | not reportedimplied by duty cycle ÷ PRF: 0.4 ms (not stated by the paper) | ⚑ |
| Pulse repetition frequency (Hz) | 1,000 | ✓✓✓ |
| Duty cycle (%) | 40 | ✓✓✓ |
| Sonication duration (s) | 0.4 | ✓✓✓ |
| Free-field pressure (kPa) | not reported | |
|---|---|---|
| Free-field Isppa (W/cm²) | not reported | |
| Free-field Ispta (W/cm²) | not reported | |
| In-situ estimate | not reported | |
| In-situ pressure (kPa) | not reported | |
| In-situ Isppa (W/cm²) | not reported | |
| In-situ Ispta (W/cm²) | not reported | |
| Isppa, domain unspecified (W/cm²) | 1.1 | ✓✓✓⚑ |
FF 500 kHz, PRF 1 kHz, SD 400 ms and DC 40%. Unfocused transducer coupled to the skull through a conical collimator.
Flags from extraction
n_subjects— 19 mice used; LIPUS n=12 and sham n=7 reported in Results.exposures[0].timing.pulse_duration_ms— Burst rule: pulse duration not stated; duty cycle 40% and PRF 1 kHz are given, SD 400 ms is the train. Left not_reported.exposures[0].unspecified_domain.isppa_w_cm2— Isppa calculated from hydrophone-measured pressure; medium not stated.sham_type— Sham group never described.exposures[0].target.terms— Target described only as visual cortex.