Seizure control by low-intensity ultrasound in mice with temporal lobe epilepsy
Xin Li, Huifang Yang, Jiaqing Yan, Xingran Wang, Yi Yuan, Xiaoli Li
Epilepsy Research 2019, 154, 1-7 · 10.1016/j.eplepsyres.2019.04.002
Abstract
Background Temporal lobe epilepsy (TLE) is the most common form of focal epilepsy. Recent studies have demonstrated that ultrasound stimulation can inhibit spontaneous recurrent seizures and improve behavioral outcomes for rodents with TLE. However, the exact underlying mechanism for inhibition of TLE via ultrasound stimulation remains unknown. Methods In this study, low-intensity pulsed ultrasound stimulation (LIPUS) and low-intensity continuous ultrasound stimulation (LICUS) and concurrent local field potentials (LFPs) in the CA3 field of the hippocampus were recorded in the kainite-induced mouse model of TLE. The power spectrum and the phase-amplitude coupling in the LFPs were quantitatively analyzed. Results We found three significant changes in LFPs after ultrasound stimulation: (i) the intensity of the power spectrum in the low frequency ( Conclusions These results indicate that the seizures of TLE can be effectively inhibited by ultrasound stimulation, and this effect is independent of ultrasound type (pulsed or continuous).
Abstract via europepmc.
Exposures
Exposure 1: LIPUS and LICUS stimulation of hippocampal CA3 in kainate-induced TLE mice
Target: CA3 — “CA3 region of the hippocampus”
Device: Olympus / Panametrics · Olympus · V301-SU
| Waveform | pulsed, continuous | |
|---|---|---|
| Fundamental frequency (kHz) | 500 | ✓✓✓ |
| Pulse duration (ms) | not reportedimplied by duty cycle ÷ PRF: 1 ms (not stated by the paper) | ⚑ |
| Pulse repetition frequency (Hz) | 500 | ✓✓✓ |
| Duty cycle (%) | 50 | ✓✓✓ |
| Sonication duration (s) | 30 | ✓✓✓ |
| Free-field pressure (kPa) | not reported | |
|---|---|---|
| Free-field Isppa (W/cm²) | not reported | |
| Free-field Ispta (W/cm²) | not reported | |
| In-situ estimate | not reported | |
| In-situ pressure (kPa) | not reported | |
| In-situ Isppa (W/cm²) | not reported | |
| In-situ Ispta (W/cm²) | not reported | |
| Pressure, domain unspecified (kPa) | 260 | ✓✓✓⚑ |
LIPUS: fundamental frequency 500 kHz, PRF 500 Hz, duty cycle 50%, stimulation duration 30 s. LICUS: continuous 500 kHz sine wave, stimulation duration 30 s. Ultrasound was manually triggered for 30 s once an LFP seizure (amplitude >3x background, lasting >10 s) was detected in the CA3 region via an unfocused transducer coupled through a conical collimator to the skull.
Flags from extraction
n_subjects— Group sizes given for the main LFP cohort (KA+LIPUS n=8, KA+LICUS n=8) and a separate latency-to-seizure cohort (KA+LIPUS n=5, KA+LICUS n=5); no single stated total exposed to ultrasound.exposures[0].unspecified_domain.pressure_kpa— Paper does not state whether the 0.26 MPa maximum pressure was measured free-field or through the skull; placed in unspecified_domain.sham_type— The KA-only group serves as an untreated comparator; no sham ultrasound (e.g. inactive transducer) mechanism is described, so classified as other.exposures[0].timing.pulse_duration_ms— Not stated directly and not computed from duty cycle/PRF per the burst rule; left not_reported.