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A study of reception with the use of focused ultrasound. I. Effects on the skin and deep receptor structures in man

Leonid R. Gavrilov, Grigoryi V. Gersuni, Oleg B. Ilyinski, Efim M. Tsirulnikov, Eugenyi E. Shchekanov

Brain Research 1977, 135, 265-277 · 10.1016/0006-8993(77)91030-7

human healthyhealthybehaviour

Abstract

The possibility of use of focused ultrasound (focused beam of high-frequency mechanical waves) for stimulation of nerve structures was investigated. The stimulation of human hand resulted in various sensations: tactile, temperature, pain etc. The corresponding thresholds were determined and characteristic features of ultrasonically induced sensations were studied. The modality of temperature sensation (warmth-cold) was found to depend on the environmental temperature. The character of pain was dependent upon the type of tissue stimulated. Effective factors in ultrasonic stimulation are discussed.

Abstract via europepmc.

Specieshuman
Subjects7 participants
Sessions per subjectnot reported
Randomisednot reported
Blindingnot reported
Sham / controlnone
Auditory controlnot reported
Readout timingonline
Anaesthesianot applicable
Readoutsbehaviourverbal report of tactile, temperature and pain sensation thresholds.
Direction of effectexcitatoryFocused ultrasound evoked tactile, thermal (warmth or cold, depending on ambient water temperature) and pain sensations by activating cutaneous and deep (soft tissue, bone, joint) receptor structures in the hand; thresholds rose moving from fingers to forearm and with deeper placement of the focal region.
Adverse eventsnot reported

Exposures

Exposure 1: Focused ultrasound to skin/deep receptor structures of the hand (480 kHz)

Target: other — “skin, soft tissue, bone and joints of the hand (fingers, palm, forearm)
Device: custom-built

Pulse timing
Waveformcontinuous
Fundamental frequency (kHz)480✓✓
Pulse duration (ms)not applicable
Pulse repetition frequency (Hz)not applicable
Duty cycle (%)not applicable
Sonication duration (s)0.001, 0.01, 0.1swept✓✓
Pressure and intensity, by domain
Free-field pressure (kPa)not reported
Free-field Isppa (W/cm²)not reported
Free-field Ispta (W/cm²)not reported
In-situ estimatenot reported
In-situ pressure (kPa)not reported
In-situ Isppa (W/cm²)not reported
In-situ Ispta (W/cm²)not reported
Isppa, domain unspecified (W/cm²)1,300✓✓
Protocol, in the paper’s words

The focusing irradiator and the subject's hand were submerged in a water bath (30, 35 or 40C); stimuli of a given duration were applied at irregular intervals with intensity rising from stimulus to stimulus, first for 1 msec, then 10 and 100 msec, until tactile, then temperature, then pain sensations appeared. The focal region could be moved from the skin surface into deeper soft tissue, bone or joint using a coordinate positioning device.

Exposure 2: Focused ultrasound to skin/deep receptor structures of the hand (887 kHz)

Target: other — “skin, soft tissue, bone and joints of the hand (fingers, palm, forearm)
Device: custom-built

Pulse timing
Waveformcontinuous
Fundamental frequency (kHz)887✓✓
Pulse duration (ms)not applicable
Pulse repetition frequency (Hz)not applicable
Duty cycle (%)not applicable
Sonication duration (s)0.001, 0.01, 0.1swept✓✓
Pressure and intensity, by domain
Free-field pressure (kPa)not reported
Free-field Isppa (W/cm²)not reported
Free-field Ispta (W/cm²)not reported
In-situ estimatenot reported
In-situ pressure (kPa)not reported
In-situ Isppa (W/cm²)not reported
In-situ Ispta (W/cm²)not reported
Isppa, domain unspecified (W/cm²)8,000✓✓
Protocol, in the paper’s words

The focusing irradiator and the subject's hand were submerged in a water bath (30, 35 or 40C); stimuli of a given duration were applied at irregular intervals with intensity rising from stimulus to stimulus, first for 1 msec, then 10 and 100 msec, until tactile, then temperature, then pain sensations appeared. The focal region could be moved from the skin surface into deeper soft tissue, bone or joint using a coordinate positioning device.

Exposure 3: Focused ultrasound to skin/deep receptor structures of the hand (2670 kHz)

Target: other — “skin, soft tissue, bone and joints of the hand (fingers, palm, forearm)
Device: custom-built

Pulse timing
Waveformcontinuous
Fundamental frequency (kHz)2,670✓✓
Pulse duration (ms)not applicable
Pulse repetition frequency (Hz)not applicable
Duty cycle (%)not applicable
Sonication duration (s)0.001, 0.01, 0.1swept✓✓
Pressure and intensity, by domain
Free-field pressure (kPa)not reported
Free-field Isppa (W/cm²)not reported
Free-field Ispta (W/cm²)not reported
In-situ estimatenot reported
In-situ pressure (kPa)not reported
In-situ Isppa (W/cm²)not reported
In-situ Ispta (W/cm²)not reported
Isppa, domain unspecified (W/cm²)30,000✓✓
Protocol, in the paper’s words

The focusing irradiator and the subject's hand were submerged in a water bath (30, 35 or 40C); stimuli of a given duration were applied at irregular intervals with intensity rising from stimulus to stimulus, first for 1 msec, then 10 and 100 msec, until tactile, then temperature, then pain sensations appeared. The focal region could be moved from the skin surface into deeper soft tissue, bone or joint using a coordinate positioning device.

Exposure 4: Focused ultrasound to thermode-defined warm/cold skin spots (1.95 MHz, one of three frequencies compared)

Target: other — “skin, soft tissue, bone and joints of the hand (fingers, palm, forearm)
Device: custom-built

Pulse timing
Waveformcontinuous
Fundamental frequency (kHz)1,950✓✓
Pulse duration (ms)not applicable
Pulse repetition frequency (Hz)not applicable
Duty cycle (%)not applicable
Sonication duration (s)0.001, 0.01swept✓✓
Pressure and intensity, by domain
Free-field pressure (kPa)not reported
Free-field Isppa (W/cm²)not reported
Free-field Ispta (W/cm²)not reported
In-situ estimatenot reported
In-situ pressure (kPa)not reported
In-situ Isppa (W/cm²)not reported
In-situ Ispta (W/cm²)not reported
Protocol, in the paper’s words

At room temperature (21-23C) a warm or cold thermode (tip diameter about 1 mm) was used to locate 'warm' and 'cold' spots on the palm skin; these same spots were then stimulated with focused ultrasound (0.887, 1.95 and 2.67 MHz; 1 and 10 msec duration; water temperature 34-36C), and the resulting cold or warmth sensation was independent of whether the spot had been classed as warm or cold by the thermode.

Flags from extraction

  • exposures[0].unspecified_domain.isppa_w_cm2Value is the transducer's stated maximal ultrasonic intensity for this frequency, not necessarily the intensity actually delivered in any given trial; per-condition threshold intensities (by water temperature, duration, location and sensation type) are given in Tables I-IV but are not individually extracted here. Domain (free-field vs at the receptor) is not stated by the paper.
  • exposures[1].unspecified_domain.isppa_w_cm2Value is the transducer's stated maximal ultrasonic intensity for this frequency, not necessarily the intensity actually delivered in any given trial; per-condition threshold intensities (by water temperature, duration, location and sensation type) are given in Tables I-IV but are not individually extracted here. Domain (free-field vs at the receptor) is not stated by the paper.
  • exposures[2].unspecified_domain.isppa_w_cm2Value is the transducer's stated maximal ultrasonic intensity for this frequency, not necessarily the intensity actually delivered in any given trial; per-condition threshold intensities (by water temperature, duration, location and sensation type) are given in Tables I-IV but are not individually extracted here. Domain (free-field vs at the receptor) is not stated by the paper.
  • exposures[3].fundamental_frequency_khz1.95 MHz is mentioned only as one of three frequencies used in a control comparison against thermode-defined warm/cold spots; no separate intensity value is given for this frequency.